On the Role of the 376-Functional Group in Catalysis by Medium Chain Acyl-CoA-dehydrogenase
نویسندگان
چکیده
c a r b o x y l a t e o f G l u 3 7 6 ( n u m b e r i n g o f h u m a n m e d i u m c h a i n a c y l C o A d e h y d r o g e n a s e , w t M C A D H ) . N e x t , i n a c o n c e r t e d p r o c e s s [ 5 , 6 ] , a h y d r i d e i s e x p u l s e d f r o m ' ~ t h e s u b s t r a t e p o s . ß a n d i s t r a n s f e r r e d t o t h e t 1 a v i n p o s . N ( 5 ) [ 7 ] . T h e r o l e o f G l u . 3 7 6 w a s d e m o n s t r a t e d b y m u t a g e n e s i s t o 3 7 6 G l n , t h i s h a v i n g ; : : ; 1 / 1 0 5
منابع مشابه
Functional role of a distal (3'-phosphate) group of CoA in the recombinant human liver medium-chain acyl-CoA dehydrogenase-catalysed reaction.
The X-ray crystallographic structure of medium-chain acyl-CoA dehydrogenase (MCAD)-octenoyl-CoA complex reveals that the 3'-phosphate group of CoA is confined to the exterior of the protein structure [approx. 15 A (1.5 nm) away from the enzyme active site], and is fully exposed to the outside solvent environment. To ascertain whether such a distal (3'-phosphate) fragment of CoA plays any signif...
متن کاملMedium-long-chain chimeric human Acyl-CoA dehydrogenase: medium-chain enzyme with the active center base arrangement of long-chain Acyl-CoA dehydrogenase.
The catalytically essential glutamate residue that initiates catalysis by abstracting the substrate alpha-hydrogen as H+ is located at position 376 (mature MCADH numbering) on loop JK in medium chain acyl-CoA dehydrogenase (MCADH). In long chain acyl-CoA dehydrogenase (LCADH) and isovaleryl-CoA dehydrogenase (IVDH), the corresponding Glu carrying out the same function is placed at position 255 ...
متن کاملThe effects of ginsenoside Rb1 on fatty acid β-oxidation, mediated by AMPK, in the failing heart
Objective(s): This study intended to investigate the effects of Ginsenoside-Rbl (Gs-Rbl) on fatty acid β-oxidation (FAO) in rat failing heart and to identify potential mechanisms of Gs-Rbl improving heart failure (HF) by FAO pathway dependent on AMP-activated protein kinase (AMPK). Materials and Methods: Rats with chronic HF, induced by adriamycin (Adr), were randomly grouped into 7 groups. Gs-...
متن کاملAcyl-CoA dehydrogenases. A mechanistic overview.
Acyl-CoA dehydrogenases constitute a family of flavoproteins that catalyze the alpha,beta-dehydrogenation of fatty acid acyl-CoA conjugates. While they differ widely in their specificity, they share the same basic chemical mechanism of alpha,beta-dehydrogenation. Medium chain acyl-CoA dehydrogenase is probably the best-studied member of the class and serves as a model for the study of catalytic...
متن کاملCharacterization of wild-type and an active site mutant of human medium chain acyl-CoA dehydrogenase after expression in Escherichia coli.
The cDNA of human medium chain acyl-CoA dehydrogenase (MCADH) was modified by in vitro mutagenesis, and the sequence encoding the mature form of MCADH was introduced into an inducible expression plasmid. We observed synthesis of the protein in Escherichia coli cells transformed with this plasmid with measurable MCADH enzyme activity in cell extracts. Glutamic acid 376, which has been proposed b...
متن کامل